GlutaredoxinV1, Main, Exploration, bibRecord, 000323

Real-Time Imaging of the Bacillithiol Redox Potential in the Human Pathogen Staphylococcus aureus Using a Genetically Encoded Bacilliredoxin-Fused Redox Biosensor.

Identifieur interne : 000323 ( Main/Exploration ); précédent : 000322; suivant : 000324

Real-Time Imaging of the Bacillithiol Redox Potential in the Human Pathogen Staphylococcus aureus Using a Genetically Encoded Bacilliredoxin-Fused Redox Biosensor.

Auteurs : Vu Van Loi [Allemagne] ; Manuela Harms [Allemagne] ; Marret Müller [Allemagne] ; Nguyen Thi Thu Huyen [Allemagne] ; Chris J. Hamilton [Royaume-Uni] ; Falko Hochgr Fe [Allemagne] ; Jan Pané-Farré [Allemagne] ; Haike Antelmann [Allemagne]

Source :

Antioxidants & redox signaling [ 1557-7716 ] ; 2017.

RBID : pubmed:27462976

Descripteurs français

KwdFr :
- Cystéine (analogues et dérivés), Cystéine (déficit), Cystéine (génétique), Cystéine (métabolisme), Facteurs temps (MeSH), Glucosamine (analogues et dérivés), Glucosamine (déficit), Glucosamine (génétique), Glucosamine (métabolisme), Oxydoréduction (MeSH), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Staphylococcus aureus (génétique), Staphylococcus aureus (métabolisme), Techniques de biocapteur (MeSH).
MESH :
- analogues et dérivés : Cystéine, Glucosamine.
- déficit : Cystéine, Glucosamine.
- génétique : Cystéine, Glucosamine, Protéines bactériennes, Protéines à fluorescence verte, Staphylococcus aureus.
- métabolisme : Cystéine, Glucosamine, Protéines bactériennes, Protéines à fluorescence verte, Staphylococcus aureus.
- Facteurs temps, Oxydoréduction, Techniques de biocapteur.

English descriptors

KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Biosensing Techniques (MeSH), Cysteine (analogs & derivatives), Cysteine (deficiency), Cysteine (genetics), Cysteine (metabolism), Glucosamine (analogs & derivatives), Glucosamine (deficiency), Glucosamine (genetics), Glucosamine (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Oxidation-Reduction (MeSH), Staphylococcus aureus (genetics), Staphylococcus aureus (metabolism), Time Factors (MeSH).
MESH :
- chemical , analogs & derivatives : Cysteine, Glucosamine.
- chemical , deficiency : Cysteine, Glucosamine.
- chemical , genetics : Bacterial Proteins, Cysteine, Glucosamine, Green Fluorescent Proteins.
- chemical , metabolism : Bacterial Proteins, Cysteine, Glucosamine, Green Fluorescent Proteins.
- genetics : Staphylococcus aureus.
- metabolism : Staphylococcus aureus.
- Biosensing Techniques, Oxidation-Reduction, Time Factors.

Abstract

AIMS

Bacillithiol (BSH) is utilized as a major thiol-redox buffer in the human pathogen Staphylococcus aureus. Under oxidative stress, BSH forms mixed disulfides with proteins, termed as S-bacillithiolation, which can be reversed by bacilliredoxins (Brx). In eukaryotes, glutaredoxin-fused roGFP2 biosensors have been applied for dynamic live imaging of the glutathione redox potential. Here, we have constructed a genetically encoded bacilliredoxin-fused redox biosensor (Brx-roGFP2) to monitor dynamic changes in the BSH redox potential in S. aureus.

RESULTS

The Brx-roGFP2 biosensor showed a specific and rapid response to low levels of bacillithiol disulfide (BSSB) in vitro that required the active-site Cys of Brx. Dynamic live imaging in two methicillin-resistant S. aureus (MRSA) USA300 and COL strains revealed fast and dynamic responses of the Brx-roGFP2 biosensor under hypochlorite and hydrogen peroxide (H

DOI: 10.1089/ars.2016.6733
PubMed: 27462976
PubMed Central: PMC5444506

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000424
to stream Main, to step Curation: 000424

Le document en format XML

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<series><title level="j">Antioxidants & redox signaling</title>
<idno type="eISSN">1557-7716</idno>
<imprint><date when="2017" type="published">2017</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term>
<term>Bacterial Proteins (metabolism)</term>
<term>Biosensing Techniques (MeSH)</term>
<term>Cysteine (analogs & derivatives)</term>
<term>Cysteine (deficiency)</term>
<term>Cysteine (genetics)</term>
<term>Cysteine (metabolism)</term>
<term>Glucosamine (analogs & derivatives)</term>
<term>Glucosamine (deficiency)</term>
<term>Glucosamine (genetics)</term>
<term>Glucosamine (metabolism)</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Oxidation-Reduction (MeSH)</term>
<term>Staphylococcus aureus (genetics)</term>
<term>Staphylococcus aureus (metabolism)</term>
<term>Time Factors (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cystéine (analogues et dérivés)</term>
<term>Cystéine (déficit)</term>
<term>Cystéine (génétique)</term>
<term>Cystéine (métabolisme)</term>
<term>Facteurs temps (MeSH)</term>
<term>Glucosamine (analogues et dérivés)</term>
<term>Glucosamine (déficit)</term>
<term>Glucosamine (génétique)</term>
<term>Glucosamine (métabolisme)</term>
<term>Oxydoréduction (MeSH)</term>
<term>Protéines bactériennes (génétique)</term>
<term>Protéines bactériennes (métabolisme)</term>
<term>Protéines à fluorescence verte (génétique)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Staphylococcus aureus (génétique)</term>
<term>Staphylococcus aureus (métabolisme)</term>
<term>Techniques de biocapteur (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Cysteine</term>
<term>Glucosamine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Cysteine</term>
<term>Glucosamine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
<term>Cysteine</term>
<term>Glucosamine</term>
<term>Green Fluorescent Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term>
<term>Cysteine</term>
<term>Glucosamine</term>
<term>Green Fluorescent Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Cystéine</term>
<term>Glucosamine</term>
</keywords>
<keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Cystéine</term>
<term>Glucosamine</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Staphylococcus aureus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cystéine</term>
<term>Glucosamine</term>
<term>Protéines bactériennes</term>
<term>Protéines à fluorescence verte</term>
<term>Staphylococcus aureus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Staphylococcus aureus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cystéine</term>
<term>Glucosamine</term>
<term>Protéines bactériennes</term>
<term>Protéines à fluorescence verte</term>
<term>Staphylococcus aureus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Biosensing Techniques</term>
<term>Oxidation-Reduction</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term>
<term>Oxydoréduction</term>
<term>Techniques de biocapteur</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en"><p><b>AIMS</b>
</p>
<p>Bacillithiol (BSH) is utilized as a major thiol-redox buffer in the human pathogen Staphylococcus aureus. Under oxidative stress, BSH forms mixed disulfides with proteins, termed as S-bacillithiolation, which can be reversed by bacilliredoxins (Brx). In eukaryotes, glutaredoxin-fused roGFP2 biosensors have been applied for dynamic live imaging of the glutathione redox potential. Here, we have constructed a genetically encoded bacilliredoxin-fused redox biosensor (Brx-roGFP2) to monitor dynamic changes in the BSH redox potential in S. aureus.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>The Brx-roGFP2 biosensor showed a specific and rapid response to low levels of bacillithiol disulfide (BSSB) in vitro that required the active-site Cys of Brx. Dynamic live imaging in two methicillin-resistant S. aureus (MRSA) USA300 and COL strains revealed fast and dynamic responses of the Brx-roGFP2 biosensor under hypochlorite and hydrogen peroxide (H</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27462976</PMID>
<DateCompleted><Year>2018</Year>
<Month>01</Month>
<Day>26</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1557-7716</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>26</Volume>
<Issue>15</Issue>
<PubDate><Year>2017</Year>
<Month>05</Month>
<Day>20</Day>
</PubDate>
</JournalIssue>
<Title>Antioxidants & redox signaling</Title>
<ISOAbbreviation>Antioxid Redox Signal</ISOAbbreviation>
</Journal>
<ArticleTitle>Real-Time Imaging of the Bacillithiol Redox Potential in the Human Pathogen Staphylococcus aureus Using a Genetically Encoded Bacilliredoxin-Fused Redox Biosensor.</ArticleTitle>
<Pagination><MedlinePgn>835-848</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1089/ars.2016.6733</ELocationID>
<Abstract><AbstractText Label="AIMS">Bacillithiol (BSH) is utilized as a major thiol-redox buffer in the human pathogen Staphylococcus aureus. Under oxidative stress, BSH forms mixed disulfides with proteins, termed as S-bacillithiolation, which can be reversed by bacilliredoxins (Brx). In eukaryotes, glutaredoxin-fused roGFP2 biosensors have been applied for dynamic live imaging of the glutathione redox potential. Here, we have constructed a genetically encoded bacilliredoxin-fused redox biosensor (Brx-roGFP2) to monitor dynamic changes in the BSH redox potential in S. aureus.</AbstractText>
<AbstractText Label="RESULTS">The Brx-roGFP2 biosensor showed a specific and rapid response to low levels of bacillithiol disulfide (BSSB) in vitro that required the active-site Cys of Brx. Dynamic live imaging in two methicillin-resistant S. aureus (MRSA) USA300 and COL strains revealed fast and dynamic responses of the Brx-roGFP2 biosensor under hypochlorite and hydrogen peroxide (H<sub>2</sub>
O<sub>2</sub>
) stress and constitutive oxidation of the probe in different BSH-deficient mutants. Furthermore, we found that the Brx-roGFP2 expression level and the dynamic range are higher in S. aureus COL compared with the USA300 strain. In phagocytosis assays with THP-1 macrophages, the biosensor was 87% oxidized in S. aureus COL. However, no changes in the BSH redox potential were measured after treatment with different antibiotics classes, indicating that antibiotics do not cause oxidative stress in S. aureus. Conclusion and Innovation: This Brx-roGFP2 biosensor catalyzes specific equilibration between the BSH and roGFP2 redox couples and can be applied for dynamic live imaging of redox changes in S. aureus and other BSH-producing Firmicutes. Antioxid. Redox Signal. 26, 835-848.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Loi</LastName>
<ForeName>Vu Van</ForeName>
<Initials>VV</Initials>
<AffiliationInfo><Affiliation>1 Institute for Biology-Microbiology, Freie Universität Berlin , Berlin, Germany .</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Harms</LastName>
<ForeName>Manuela</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>2 Junior Research Group Pathoproteomics, Ernst-Moritz-Arndt-University of Greifswald , Greifswald, Germany .</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Müller</LastName>
<ForeName>Marret</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>3 Institute for Microbiology, Ernst-Moritz-Arndt-University of Greifswald , Greifswald, Germany .</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Huyen</LastName>
<ForeName>Nguyen Thi Thu</ForeName>
<Initials>NTT</Initials>
<AffiliationInfo><Affiliation>1 Institute for Biology-Microbiology, Freie Universität Berlin , Berlin, Germany .</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hamilton</LastName>
<ForeName>Chris J</ForeName>
<Initials>CJ</Initials>
<AffiliationInfo><Affiliation>4 School of Pharmacy, University of East Anglia , Norwich Research Park, Norwich, United Kingdom .</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hochgräfe</LastName>
<ForeName>Falko</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>2 Junior Research Group Pathoproteomics, Ernst-Moritz-Arndt-University of Greifswald , Greifswald, Germany .</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pané-Farré</LastName>
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<Initials>J</Initials>
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Real-Time Imaging of the Bacillithiol Redox Potential in the Human Pathogen Staphylococcus aureus Using a Genetically Encoded Bacilliredoxin-Fused Redox Biosensor.

Real-Time Imaging of the Bacillithiol Redox Potential in the Human Pathogen Staphylococcus aureus Using a Genetically Encoded Bacilliredoxin-Fused Redox Biosensor.

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